Coding

Part:BBa_K4162003:Design

Designed by: Yujian Song   Group: iGEM22_Fudan   (2022-08-21)


ybbO coding for retinol dehydrogenase, codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
    Illegal AgeI site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

codon-optimized


Source

The ybbO gene is reported to have transferred from an eukaryotic retinol dehydrogenase ancestor to the E. coli genome in the human intestine via horizontal gene transfer, as shown by sequence comparison and phylogenetic analysis.

References